Seyed Mohammad Mousavi; Amin Tabibi; Vahid Yavari; Mohammad Zakeri; Arash Jahedi; Hossein Pasha Zanoosi
Volume 5, Issue 3 , March 2017, , Pages 39-50
Abstract
Gatan has distributed in the main rivers in Khoozestan province and there are few researches on propagation and reproductive system of this species. Therefore, this study has focused on using of activating solutions on sperm activity and fertilization capability in Gatan fish in comparison with Grass ...
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Gatan has distributed in the main rivers in Khoozestan province and there are few researches on propagation and reproductive system of this species. Therefore, this study has focused on using of activating solutions on sperm activity and fertilization capability in Gatan fish in comparison with Grass Carp. In this research, 5 activating solutions together with workshop water and distilled water were used in triplicate. After transferring of brood stocks to the propagation workshop and after hormontherapy, sperm and ovules were collected. Assessment of sperm activity was done and after mixing of sperm and ovules, activating solutions were used. After 12 hours of fertilization time, fertilization percentage was assessed. Based on results obtained from this study, the highest sperm activity and fertilization rate was seen in Gatan fish when a2 (Tris Hcl 20 mM, NaCl 50mM), a3 (CaCl2 2.5mM) and a4 (Tris Hcl 30 mM, KCl 30mM, NaCl 50mM) solutions were used and lowest sperm activity was seen when workshop water and distilled water were used in this species (P<0.001). Also, the highest sperm activity and fertilization rate was seen in Grass Carp when a2, a4 and 4030 solutions were used (P<0.001). Based on the results, finally it is revealed that the highest sperm activity and fertilization per cent among activating solutions has resulted from a2 activating solution combining from NaCl and Tris-Hcl in both fishes examined in this study which was led to increase of around seven times in sperm activity and around 20% in fertilization per cent.
